Thermo Scientific™ Phire Plant Direct PCR Master Mix PROMO

Amplify DNA directly from plant samples without prior DNA purification with Phire Plant Direct PCR Master Mix.

Supplier:  Thermo Scientific™ F160S

Catalog No. F160SPM

Description

Highlights

• Direct PCR - sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps.
• Dilution and Storage protocol available - allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire Hot Start II DNA polymerase - extremely short PCR protocol times
• Master Mix format with premixed gel loading dye - minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Includes

• 2x Phire Plant Direct PCR Master Mix
• Dilution Buffer
• Control Primer Mix (25 μM each)
• Water, nuclease- free
• O'GeneRuler Express DNA Ladder

Specifications

• Content And Storage: Contains: 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X), 5 mL Dilution Buffer, 40 μL Control Primer Mix (25 μM each), 2 x 1.25 mL Water, nuclease- free, 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications), Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Line: Phire
• Quantity: 250 x 50 μL Reactions
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Plant

• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA polymerase
• Includes Label or Dye: Yes
• Product Type: Direct PCR Master Mix
• For Use With (Application): Genotyping
• No. of Reactions: 250 x 50 L Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.