Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Thermo Scientific™ Phire Tissue Direct PCR Master Mix PROMO
Amplify DNA directly from variety of human and animal samples with no prior purification with Phire Tissue Direct PCR Master Mix.
Specifications
| GC-Rich PCR Performance | Medium |
|---|---|
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA polymerase |
| Reaction Speed | Fast |
| Includes Label or Dye | Yes |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|
F170SPM
|
Thermo Scientific™
F170S |
250 x 20 μL Reactions | N/A | N/A | N/A | ||||
Description
The Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.
Highlights
- Direct PCR – sample is added directly to PCR reaction, therefore therese is no need for time-consuming and expensive DNA purification steps
- Dilution and Storage protocol available – allows tens of PCR reactions from one tiny sample and allows re-testing
- Specially engineered Phire DNA polymerase – extremely short PCR protocol times
- Master Mix format with premixed gel loading dye – minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
Includes
- 2X Phire Tissue Direct PCR Master Mix
- Reaction voume with dilution and storage protocol: 20 μL
- Reaction voume with direct protocol: 50 μL
- Dilution Buffer
- DNARelease Additive
- Universal control primer mix (25 μM each)
- Water, nuclease- free
- O'GeneRuler Express DNA Ladder
Specifications
| Medium | |
| Phire Hot Start II DNA polymerase | |
| Yes | |
| Direct PCR Master Mix | |
| Built-In Hot Start | |
| SuperMix or Master Mix | |
| DNA |
| Direct PCR | |
| Fast | |
| Phire | |
| Genotyping | |
| Blunt | |
| Tissue |
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title