Phospho-AKT (Thr308) Mouse anti-Human, Monkey, Mouse, Rat, Biotin, Clone: 18F3.H11, Rockland™

Catalog No.	Quantity
50-199-0700	50 μg

Catalog No. 50-199-0700 Supplier Rockland Immunochemicals Supplier No. 200306269

Description

Mouse Monoclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

Specifications

• Antigen: Phospho-AKT (Thr308)
• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 18F3.H11
• Concentration: 1 mg/mL
• Conjugate: Biotin
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Gene: AKT1
• Gene Accession No.: P31749, P31750, P47196
• Gene Alias: Akt; Akt kinase; AKT serine/threonine kinase 1; AKT/PKB; AKT1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; AKT2; AKT3; CAKT; C-AKT; CG4006; CG4006 gene product from transcript CG4006-RE; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; DAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAkt1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; kinase Akt1; l(3)04226; l(3)89Bq; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; p-Akt; Phospho-AKT; Phospho-PKB; PKB; PKB Akt; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; Rac; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; serine/threonine protein kinase; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
• Gene Symbols: AKT1
• Host Species: Mouse
• Immunogen: Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11651, 207, 24185
• Target Species: Human, Monkey, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG1 κ