Invitrogen™ Phospho-PAK4/6/7 (Ser474) Polyclonal Antibody

Catalog No.	Quantity
PIPA536865	100 μL

Catalog No. PIPA536865 Supplier Invitrogen™ Supplier No. PA536865

Description

Rabbit Polyclonal Antibody

This antibody detects endogenous protein at a molecular weight of 72 kDa (PAK4), 82 kDa (PAK6) and 90 (PAK7). Purity is >95% by SDS-PAGE.

PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.

Specifications

• Antigen: Phospho-PAK4/6/7 (Ser474)
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene: Pak4
• Gene Accession No.: O96013, Q9NQU5, Q9P286
• Gene Alias: 2900083L08Rik; 4732456M09; 5730488L07Rik; 6430627N20; AW555722; KIAA1142; KIAA1264; mKIAA1142; p21 (CDKN1A)-activated kinase 4; p21 (CDKN1A)-activated kinase 6; p21 (CDKN1A)-activated kinase 7; p21 (RAC1) activated kinase 4; p21 (RAC1) activated kinase 5; p21 (RAC1) activated kinase 6; p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 4; p21(CDKN1A)-activated kinase 6; p21(CDKN1A)-activated kinase 7; p21-activated kinase 4; p21-activated kinase 5; p21-activated kinase 6; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK 4/5/6; PAK 4/6/7; PAK4; PAK-4; PAK4/5/6; PAK5; PAK-5; PAK6; PAK-6; Pak7; PAK-7; protein kinase PAK5; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; serine/threonine-protein kinase PAK 4; Serine/threonine-protein kinase PAK 5; serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK 6-like; serine/threonine-protein kinase PAK 7; serine/threonine-protein kinase PAK7
• Gene Symbols: Pak4, PAK5, PAK6
• Host Species: Rabbit
• Immunogen: Synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Serine 474.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10298, 56924, 57144
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG