Phospho-RNA pol II CTD (Ser2) Monoclonal Antibody (3E10), Active Motif™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-3123	Active Motif 61084	10 μg
50-199-3124	Active Motif 61984	50 μg
50-199-3120	Active Motif 61083	100 μg

Description

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

Specifications

• Antigen: Phospho-RNA pol II CTD (Ser2)
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
• Gene Alias: 220kDa; AW321876; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha 1, 180 kDa; DNA polymerase alpha 1, catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DNA polymerase alpha subunit I; DNA polymerase epsilon catalytic subunit; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DUN2; EC 2.7.7.6; G7a/Bat6Hom; hRPB220; hsRPB1; I79_002751; I79_016081; MGC75453; N0825; NSX; p180; Pol II S2p; POL2; Pola; POLA1; POLR2; Polr2a; POLRA; polymerase (DNA directed), alpha 1; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); Polymerase (RNA II (DNA directed), large polypept
• Gene Symbols: POLA1, POLR2A
• Isotype: IgG1
• Purification Method: Protein G
• Product Type: Antibody

• Clone: 3E10
• Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Human, Mouse
• Gene Accession No.: P08775, P09884, P24928, P33609
• Gene ID (Entrez): 18968, 20020, 5422, 5430
• Immunogen: This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: POLA1