Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

$432.00

Specifications

Antigen Phospho-S6 (Ser235, Ser236)
Clone cupk43k
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
View More Specs

Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-4219
Encompass_Preferred
View Documents
Invitrogen™
48900742
100 Tests
Each of 1 for $432.00
Only null left
Edge
Add to Cart
 
Description

Description

Description: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse). Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens. Phosphorylation of ribosomal protein S6 by p70S6K and PKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry. Ribosomal protein S6 is dephosphorylated upon growth arrest. The specificity of the cupk43k monoclonal antibody was determined by western blotting. Applications Reported: This cupk43k antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This cupk43k antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Specifications

Specifications

Phospho-S6 (Ser235, Ser236)
5 μL/Test
Monoclonal
Liquid
RUO
P62753, P62754
RPS6
Primary
4°C, store in dark, DO NOT FREEZE!
RPS6
cupk43k
Flow Cytometry
eFluor 450
Mouse
Human, Mouse
20104, 6194
IgG1 κ
Affinity Chromatography
Antibody
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.