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Phosphotyrosine Monoclonal Antibody (pY20), HRP, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 037720

Encompass_Preferred

Catalog No. 03-772-0


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Description

Description

03-7720 has been successfully used in ELISA and WB applications, and detects proteins which contain phosphorylated tyrosine residues and proteins containing phosphorylated threonine residues.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Phosphotyrosine
Monoclonal
0.5 mg/mL
PBS with 40% glycerol, 4mg/mL BSA and 0.1% ProClin 300; pH 7.4
Mouse
purified
RUO
4° C
Liquid
Chemical
ELISA, Western Blot
pY20
HRP
Phosphotyrosine; pTyr; pY
Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine.
100 μg
Primary
Antibody
IgG2b
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