Thermo Scientific™ Phusion Buffers 

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases.

Manufacturer:  Thermo Scientific™ F518L

Catalog No. F518L

Description

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer and 5X Phusion GC Buffer. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10^-7) is lower than that in GC Buffer (9.5 × 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

HF Buffer for Phusion DNA Polymerases Suitable for all standard templates

Includes:

5x Phusion HF Buffer, 50 mM MgCl₂ solution, 100% DMSO.

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Specifications

• Description: Phusion™ HF Buffer Pack
• For Use With (Application): Phusion DNA polymerases.
• Quantity: 6mL
• Storage Requirements: Store at -20°C. Stable for one year from the packaging date.