Learn More
Thermo Scientific Phusion Buffers
Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases.
Manufacturer: Thermo Scientific F518L
Description
Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer and 5X Phusion GC Buffer. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
HF Buffer for Phusion DNA Polymerases Suitable for all standard templatesIncludes:
5x Phusion HF Buffer, 50 mM MgCl2 solution, 100% DMSO.
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
Specifications
Phusion™ HF Buffer Pack | |
Phusion DNA polymerases. | |
6mL | |
Store at -20°C. Stable for one year from the packaging date. |
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.