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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase PROMO

A gold standard for high performance PCR.

Specifications

Content And Storage Phusion DNA Polymerase (2 U/μL), 5X Phusion HF GC Buffers, DMSO, 50 mM MgCl2 Solution, Phusion HF Buffer and Phusion GC Buffers Each Provide 1.5 mM MgCl2 in the Final 1X Concentration. Store at -20°C.
GC-Rich PCR Performance High
Polymerase Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
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Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
F530LW25
View Documents
Thermo Scientific™
F530L
500 Reactions N/A N/A N/A
F530SW25
View Documents
Thermo Scientific™
F530S
100 Reactions N/A N/A N/A
Description

Description

Features of Phusion™ High-Fidelity DNA Polymerase (2 U/μL) include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

Specifications

Phusion DNA Polymerase (2 U/μL), 5X Phusion HF GC Buffers, DMSO, 50 mM MgCl2 Solution, Phusion HF Buffer and Phusion GC Buffers Each Provide 1.5 mM MgCl2 in the Final 1X Concentration. Store at -20°C.
Phusion High-Fidelity DNA Polymerase
High-Fidelity DNA Polymerase
Standard PCR, High-fidelity PCR
52X
Blunt
20 kb or less
High
Fast
Dry Ice
2 U/μL
No
Standalone
Colorless
Videos
SDS
Documents

Documents

Product Certifications
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