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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase PROMO
A gold standard for high performance PCR.
Specifications
Content And Storage | Phusion DNA Polymerase (2 U/μL), 5X Phusion HF GC Buffers, DMSO, 50 mM MgCl2 Solution, Phusion HF Buffer and Phusion GC Buffers Each Provide 1.5 mM MgCl2 in the Final 1X Concentration. Store at -20°C. |
---|---|
GC-Rich PCR Performance | High |
Polymerase | Phusion High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Product Type | High-Fidelity DNA Polymerase |
Description
Features of Phusion™ High-Fidelity DNA Polymerase (2 U/μL) include:
- High fidelity (52X Taq)
- Fast PCR due to short extension times (15–30 s/kb)
- Robust performance, minimal optimization needed
- High yields of PCR products with minimal enzyme amounts
- Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)
Applications
- High-fidelity PCR
- Cloning
- Template generation for sequencing
- Amplification of difficult (GC-rich) templates
- Long-range PCR (up to 20 kb)
- Mutagenesis
- High throughput PCR
- Microarray
Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.
Specifications
Phusion DNA Polymerase (2 U/μL), 5X Phusion HF GC Buffers, DMSO, 50 mM MgCl2 Solution, Phusion HF Buffer and Phusion GC Buffers Each Provide 1.5 mM MgCl2 in the Final 1X Concentration. Store at -20°C. | |
Phusion High-Fidelity DNA Polymerase | |
High-Fidelity DNA Polymerase | |
Standard PCR, High-fidelity PCR | |
52X | |
Blunt | |
20 kb or less |
High | |
Fast | |
Dry Ice | |
2 U/μL | |
No | |
Standalone | |
Colorless |
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