Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase PROMO

A gold standard for high performance PCR.

Catalog Number	Mfr. No.	No. of Reactions
F530LW25	Thermo Scientific™ F530L	500 Reactions
F530SW25	Thermo Scientific™ F530S	100 Reactions

Description

Features of Phusion™ High-Fidelity DNA Polymerase (2 U/μL) include:

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

• Content And Storage: Phusion DNA Polymerase (2 U/μL), 5X Phusion HF GC Buffers, DMSO, 50 mM MgCl₂ Solution, Phusion HF Buffer and Phusion GC Buffers Each Provide 1.5 mM MgCl₂ in the Final 1X Concentration. Store at -20°C.
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Type: High-Fidelity DNA Polymerase
• For Use With (Application): Standard PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• Overhang: Blunt
• Size (Final Product): 20 kb or less

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Concentration: 2 U/μL
• Hot Start: No
• Reaction Format: Standalone
• Color: Colorless