Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Description

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

• Concentration: 2 U/μL
• Content And Storage: • Phusion DNA Polymerase (2 U/μL)
  • 5X Phusion HF & GC Buffers
  • DMSO
  • 50 mM MgCl₂ solution
  
  Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration.
  Store at -20°C.
• GC-Rich PCR Performance: High
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Reaction Speed: Fast
• Product Type: High-Fidelity DNA Polymerase
• Quantity: 500 units
• Shipping Condition: Dry Ice
• For Use With (Application): Standard PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• Hot Start: No
• No. of Reactions: 500 Reactions
• Overhang: Blunt
• Reaction Format: Standalone
• Size (Final Product): 20 kb or less
• Color: Colorless

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.