Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Manufacturer:  Thermo Scientific™ 34000

Catalog No. PI34000


Add to cart

Description

Description

Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.

This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.

Highlights:

  • HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)

  • Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph

  • Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve

  • Stable – store refrigerated at 4°C for at least one year

Includes:

10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer

Specifications

Specifications

Pierce™
Colorimetric
Sufficient For: 25 uses at 10 mL each
• CN/DAB (10X), 25 mL
• Stable Peroxide Buffer, 250 mL

Store at 4°C.
Chemical Substrate
HRP (Horseradish Peroxidase) Substrate
SDS
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit