Thermo Scientific Pierce CN/DAB Substrate Kit
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
Manufacturer: Thermo Scientific 34000
Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.
HRP substrate for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)
Chromogenic no special equipment needed for visualization; yields intense black bands that are easy to photograph
Easy-to-use kit substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve
Stable store refrigerated at 4°C for at least one year
10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer
|Sufficient For: 25 uses at 10 mL each
• CN/DAB (10X), 25 mL
• Stable Peroxide Buffer, 250 mL
Store at 4°C.
|HRP (Horseradish Peroxidase) Substrate|
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