Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Supplier:  Thermo Scientific™ 32209

Catalog No. PI32209

Description

Thermo Scientific™ Pierce ECL Western Blotting Substrate is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard enhanced chemilumescent (ECL) substrates for detection of horseradish peroxidase (HRP) enzyme activity, yet costs much less. Because the luminol and peroxide reagent-formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Simply switch to Pierce ECL and save a bundle.

Highlights:

• ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

• About half the cost of other ECL Substrates – low overhead and a commitment to customer value enables Thermo Fisher Scientific to make this product available to you at a significantly lower price than other companies (these claims are based on the 2010 U.S. list prices)

• No optimization required – switching to Pierce ECL substrate from other entry-level ECL substrates does not require optimization or protocol changes

• A name you can can rely on – count on the strong quality assurance, technical support and reputation behind Thermo Scientific Pierce Protein Research Products

Includes:

Detection Reagent 1 and 2

Specifications

• Product Type: Substrate
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 250 mL
• Recommended Antibody Concentrations: 2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)
• Signal Duration: 1 to 2 hr.
• Content And Storage: Upon receipt store at 2-8°C. Product is shipped at ambient temperature.

• Detection Method: Chemiluminescent
• For Use With (Application): Western Blot
• Product Line: Pierce™
• Substrate: Horseradish Peroxidase
• Sensitivity: Low Picogram
• Sufficient For: 2500 sq. cm

For Research Use Only. Not for use in diagnostic procedures.