Thermo Scientific™ Pierce™ Recombinant Protein A, Peroxidase Conjugated PROMO

Description

Detect rabbit and human antibodies, especially IgG isotypes, in Western blot, ELISA and other immunoassays with this purified and peroxidase-conjugated recombinant Protein A.

Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. coli and functions essentially the same as native Protein A. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6).

Highlights:

• Contains four Fc-binding domains per protein
• Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum
• Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies

Specifications

• Product Type: Protein A, Peroxidase Conjugate
• Conjugate: HRP
• Quantity: 1 mg
• Product Line: Pierce™
• Form: Powder
• Protein Subtype: Protein A
• Species: Human, Rabbit
• Target: Antibodies
• Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area.

For Research Use Only. Not for use in diagnostic procedures.