Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

Description

Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.

Thermo Scientific™ Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Highlights:

• Water-soluble
• Cleavable at pH 8.5 using hydroxylamine and heat
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking
• Reactive groups: sulfo-NHS esters (homobifunctional)
• Reactive toward: amino groups

Specifications

• Chemical Reactivity: Amine-Amine
• Cleavable: By Hydroxylamine
• Description: Sulfo-EGS
• Molecular Weight (g/mol): 660.45
• PEGylated: No
• Spacer Arm Length: 16.1 Å
• Content And Storage: Upon receipt store desiccated at -20°C.
• Cell Permeability: No
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Homobifunctional
• Reactive Moiety: Sulfo-NHS Ester
• Spacer: Medium (10 to 30 Å)
• Form: Powder
• Quantity: 50 mg
• Solubility: Water
• Format: Standard
• Water Soluble: Yes
• Product Type: Crosslinker

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.