Novus Biologicals™ PLA2G1B Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse PLA2G1B.

Specifications

• Assay Range: 3.125 pg/mL
• Assay Sensitivity: 3.125-200 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse PLA2G1B.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: Group IB phospholipase A2, Phosphatidylcholine 2-acylhydrolase 1B, phospholipase A2, phospholipase A2, group IB (pancreas), PLA2AMGC119834, PLA2EC 3.1.1.4, PPLA2MGC119835
• Gene ID (Entrez): 5319
• Gene Symbol: PLA2G1B
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 12 ng of recombinant Mouse PLA2G1B.
  Capture Antibody: 1 mg/mL of rabbit anti-Mouse PLA2G1B monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-PLA2G1B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Lipid and Metabolism
• Storage Requirements: Storage is content dependent.
• Target: PLA2G1B
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 200 pg/mL is recommended.