PMEL17/SILV Antibody (HMB45), Novus Biologicals™

Catalog No.	Quantity
NBP24452000	0.02 mg
NBP24452001	0.1 mg
NBP24452002	0.2 mg

Catalog No. NBP24452000 Supplier Novus Biologicals Supplier No. NBP2445200.02MG

Description

Mouse Monoclonal Antibody has been used in 3 publications

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

PMEL17/SILV Monoclonal specifically detects PMEL17/SILV in Human, Canine (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular), Immunofluorescence, Multiplex Immunoassay.

Specifications

• Antigen: PMEL17/SILV
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Classification: Monoclonal
• Clone: HMB45
• Concentration: 0.2mg/mL
• Conjugate: Unconjugated
• Dilution: Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:50 - 1:100
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
• Gene Symbols: PMEL
• Host Species: Mouse
• Immunogen: Extract of pigmented melanoma metastases from lymph nodes
• Molecular Weight of Antigen: 95 kDa
• Purification Method: Protein A or G purified
• Quantity: 0.02 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 6490
• Test Specificity: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
• Target Species: Human, Canine (Negative), Rat (Negative)
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only