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Invitrogen™ PolarScreen™ ER Alpha Competitor Assay, Green Non-distribution product as customer accommodation.

PolarScreen™ ER Alpha Competitor Assay, Green

Manufacturer:  Invitrogen™ A15882

Catalog No. A15882

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The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands. The kit uses insect cell-expressed, full-length, native (untagged) human estrogen receptor alpha (available separately, Cat. No. A15674) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone ES2 Green) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact

PolarScreen™ ER Alpha Competitor Assay, Green, is:

  • Complete & ready-to-use out of the box—all reagents included, just add your test compounds
  • High throughput compatible—800 x 20µL fluorescence polarization assays in a 384-well plate
  • Convenient—available in bulk sizes for larger screening needs

    How the Assay Works
    Full length ER alpha is added to a fluorescent estrogen ligand to form an ER-Fluormone ES2 complex. This complex is added to individual test compounds in multiwell plates. To determine relative affinity, the assay measures fluorescence polarization. Competing test compounds will displace the Fluormone ES2 ligand from ER alpha, permitting it to tumble rapidly and resulting in low polarization values. A shift in the fluorescence polarization value in the presence of test compound is used to determine the relative affinity of test compounds for ER alpha.

    All components are manufactured under the strictest quality parameters. This product's performance, per the Certificate of Analysis, is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
  • Specifications


    PolarScreen™ ER Alpha Competitor Assay Kit, 2500 Assays, 384-Well Plate Format, Fluorescent Detection Method, For Pharma & biopharma, target & lead identification & validation
    Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor binding
    384-well plate
    PolarScreen™ ER Alpha Competitor Assay Kit
    2500 Assay(s)
    2500 Assay(s)
    Fluorescent imager
    4 Tubes full-length ER alpha recombinant protein, 1 tube of fluormone ES2 green tracer, 2 bottles of ES2 screening buffer
    PolarScreen™ ER Alpha Competitor Assay Kit
    For research use only
    –68 to –85/–5 to –30°C
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    For Research Use Only. Not for use in diagnostic procedures.