Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB151708	Novus Biologicals NBP23652325UL	25 μL
NBP236523	Novus Biologicals NBP236523	0.05 mL

Description

Poly-Ubiquitin Monoclonal antibody specifically detects Poly-Ubiquitin in Nonspecies specific samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

• Antigen: Poly-Ubiquitin
• Dilution: Western Blot 1:500 - 1:1000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Formulation: PBS with 0.09% Sodium Azide
• Gene ID (Entrez): 6233
• Isotype: IgG2a
• Purification Method: Ion exchange chromatography

• Clone: HWA4C4
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Non-species specific
• Gene Alias: CEP80, HEL112, ribosomal protein S27a, RPS27A, S27A, UBA80, UBC, UBCEP1, UBCEP80
• Immunogen: The hybridoma secreting the antibody was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif.
• Primary or Secondary: Primary
• Test Specificity: Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.

For Research Use Only