Invitrogen™ PP2A alpha Monoclonal Antibody (3B6)

Catalog No.	Quantity
PIMA549213	100 μg

Catalog No. PIMA549213 Supplier Invitrogen™ Supplier No. MA549213

Description

Mouse Monoclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha. Positive Control - WB: human HELA whole cell, human HEPG2 whole cell, monkey COS-7 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell. IHC: human mammary cancer tissue, human rectal cancer tissue, rat brain tissue. ICC/IF: HELA cell. Flow: U937 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

Specifications

• Antigen: PP2A alpha
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 3B6
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and no preservative
• Gene: PPP2CA
• Gene Accession No.: P63330, P63331, P67775
• Gene Alias: B55-GAMMA; hypothetical protein; IMYPNO; IMYPNO1; Methyl-PPP2CA; MGC33570; PP2A; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; Pp2a1; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; Ppp2ca; PR52; PR55G; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2 catalytic subunit alpha; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2,catalytic subunit alpha isozyme; protein phosphatase 2A alpha subunit; protein phosphatase 2A catalytic subunit alpha isoform; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase type 2A catalytic subunit; protein phosphatase-2A; protein phosphatase-2A-alpha; R75353; replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; unnamed protein product
• Gene Symbols: PPP2CA
• Host Species: Mouse
• Immunogen: E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19052, 24672, 5515
• Target Species: Human, Mouse, Monkey, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a