Invitrogen™ pro-Caspase 3 Monoclonal Antibody (31A893)

Catalog No.	Quantity
01-676-479	100 μg

Catalog No. 01-676-479 Supplier Invitrogen™ Supplier No. MA141163

Description

Mouse Monoclonal Antibody

Suggested positive control: HL-60 or staurosporine treated apoptotic HeLa.

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Specifications

• Antigen: pro-Caspase 3
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 31A893
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: CASP3
• Gene Accession No.: P42574
• Gene Alias: A830040C14Rik; AC-3; Apopain; Casp3; CASP-3; caspase 3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; CC3; Cpp32; CPP-32; CPP32B; cysteine protease CPP32; LICE; mldy; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1; Yama
• Gene Symbols: CASP3
• Host Species: Mouse
• Immunogen: Recombinant full-length human Caspase-3 protein.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 836
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG