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Promega Exonuclease III

Catalog No. PRM1811 Shop All Promega Corporation Products
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5000 U
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PR-M1811 5000 U
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Catalog No. PR-M1811 Supplier Promega Supplier No. M1811
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Description

Includes

  • Part No. M1811: Exonuclease III 10X Buffer (1 x 1mL); Exonuclease III (5000U).
  • Part No. M1815: Exonuclease III 10X Buffer (5 x 1mL); Exonuclease III (25,000U).

Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.

  • A 3′→5′ Exonuclease Specific for Double-Stranded DNA
  • Control deletion rate by varying incubation temperature
  • Inactivate Exonuclease III by heating at 75°C for 10 minutes
  • Supplied at a concentration of 150-200u/μL

Specifications

Concentration 150 to 200 U/μL
Color Colorless
Components 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
Purity >90%
pH 7.5
For Use With (Application) Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Storage Buffer 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 5000 U
Product Type Exonuclease III
Molecular Weight (g/mol) 28000
Inhibitors 50mM NaCl
Form Liquid
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