Promega Exonuclease III

Description

Includes

• Part No. M1811: Exonuclease III 10X Buffer (1 x 1mL); Exonuclease III (5000U).
• Part No. M1815: Exonuclease III 10X Buffer (5 x 1mL); Exonuclease III (25,000U).

Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.

• A 3′→5′ Exonuclease Specific for Double-Stranded DNA
• Control deletion rate by varying incubation temperature
• Inactivate Exonuclease III by heating at 75°C for 10 minutes
• Supplied at a concentration of 150-200u/μL

Specifications

• Concentration: 150 to 200 U/μL
• Color: Colorless
• Components: 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
• Purity: >90%
• pH: 7.5
• For Use With (Application): Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
• Storage Buffer: 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Recombinant E. coli strain
• Quantity: 5000 U
• Product Type: Exonuclease III
• Molecular Weight (g/mol): 28000
• Inhibitors: 50mM NaCl
• Form: Liquid