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  6. Promega GeneClip™ U1 Hairpin Cloning Systems

Promega GeneClip™ U1 Hairpin Cloning Systems

Catalog No. PRC8790
Click to view available options
Vector:
Hygromycin
Puromycin
hMGFP

Description

Includes

2X Rapid Ligation Buffer, 200μL; Nuclease-free Water, 1.25mL; Oligo Annealing Buffer, 1mL; pGeneClip Vector (varies with system), 1.2μg; T4 DNA Ligase, 100U

Designed for fast and easy cloning of human hairpin target sequences to allow expression of siRNA target sequences in human cells

Use pGeneClip™ vectors containing the human U1 promotor, which allows transcription of the hairpin target sequences and generation of hairpin siRNA in vivo.

  • Utilize predigested vectors to eliminate time-consuming vector preparation
  • Allow easier identification of desired clones using a Pst I digestion

  • Available with a choice of vectors
    • The hMGFP System enables easy determination of transfection efficiency
    • The other systems offer antibiotic marker options for selecting stably transfected eukaryotic cells so that experimental results do not depend on transfection efficiency

  • For nonprofit and noncommercial research use only. With respect to commercial research use or use by for-profit organizations, or any diagnostic or therapeutic uses, please contact Promega Corporation for supply and licensing information.
  • Licensed under U.S. Patent 6,573,099 and its foreign equivalents from Benitec Australia Ltd and CSIRO.
  • This product is covered under license from Carnegie Institution of Washington under U.S. Pat. Nos. 6,506,559, 7,538,095, 7,560,438; Australian Patent 743798 and other patents pending. Commercial use of this product may require a separate license from Carnegie.
  • For research use only. With respect to any and all other uses, please contact Promega Corporation for supply and licensing information.
  • Certain applications of this product may require licenses from others.

Specifications

For Use With (Application) Fast and easy cloning of Human hairpin target sequences to allow expression of siRNA target sequences in Human cells
Includes 2X Rapid Ligation Buffer, 200μL; Nuclease-free Water, 1.25mL; Oligo Annealing Buffer, 1mL; pGeneClip hMGFP Vector, 1.2μg; T4 DNA Ligase, 100U
No. of Reactions 20
pH 7.4
Promoter U1
Vector hMGFP
Form Liquid
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