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Promega GoTaq™ 1-Step RT-qPCR System for Dye-Based Detection

Catalog No. PRA6020 Shop All Promega Corporation Products
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PRA6020 200 Reactions
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Catalog No. PRA6020 Supplier Promega Supplier No. A6020
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Description

For quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol for dye-based detection.

  • Convenient Master Mixes for Quantitative Real-Time PCR and RT-qPCR
  • Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Specifications

Content And Storage 2X qPCR Master mix (5 x 1mL), RT Mix (1 x 225μL), CXR reference Dye (1 x 200μL), MgCl2 (1 x 750μL), Nuclease free Water (2 x 13mL). Store at −30 to −10°C
Detection Method SYBR
Form Liquid
Format Kit
PCR Method 1-step RT-qPCR
Polymerase GoTaq™ DNA Polymerase
Reaction Speed Fast or Standard
Technique RT-qPCR
For Use With (Equipment) Analytik Jena qTOWERiris PCR thermal cycler
High-throughput Compatibility High-throughput Compatible
Includes Label or Dye Yes
Kit Type 1-Step RT-qPCR system
Label or Dye SYBR Green
No. of Tests 200
Passive Reference Dye CXR (Separate Tube)
Product Line GoTaq
Product Type RT-qPCR Kit
Quantity 5mL
Research Category Gene Expression Analysis
Reverse Transcriptase GoScript™
Sufficient For 500 Reactions of 20 μL
For Use With (Application) Real Time PCR (qPCR)
Final Product Type cDNA
Hot Start Built-In Hot Start
No. of Reactions 500 Reactions
Reaction Format Separate Components
Reagent Type RT-qPCR, Reverse Transcription
Sample Type Blood
Starting Material RNA
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