Promega GoTaq™ 2-Step RT-qPCR System

Catalog No.
PRA6010	50 RT reactions + 200 qPCR reactions

Catalog No. PRA6010 Supplier Promega Supplier No. A6010

Description

Includes

GoTaq QPCR Master Mix, 2X; GoScript Reverse Transcriptase; GoScript Reaction Buffer, 5X; CXR Reference Dye, 100X; Recombinant RNasin Ribonuclease Inhibitor; Random Primers; PCR Nuceotide Mix; Oligo (dT)₁₅; MgCl₂; Nuclease-free Water

Combines the ultra-active GoScript™ Reverse Transcriptase with the ultra-bright fluorescence of GoTaq qPCR Master Mix to provide optimized, sensitive quantification of a full range of RNA targets

• Convenient Master Mixes for Quantitative Real-Time PCR and RT-qPCR
• Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
• Compatible with both fast and standard cycling methods
• Resistant to a wide range of PCR inhibitors

Specifications

• Content And Storage: 2X QPCR Master mix (5 x 1mL), GoScript™ reverse Transcriptase (1 x 50μL), 5X GoScript™ reaction Buffer (1 x 300μL), 100X CXR Reference Dye (1 x 100μL), Recombinant R. Store at −30 to −10°C
• Detection Method: qPCR, RT-PCR
• Form: Liquid
• Format: Kit
• PCR Method: 2-step RT-qPCR
• Polymerase: GoTaq™ Hot Start Polymerase
• Technique: RT-qPCR, Reverse Transcription
• DNase: DNase-free
• For Use With (Equipment): RT- PCR Instrumen Detecting SYBR Green I or FAM Dye
• High-throughput Compatibility: High-throughput Compatible
• Includes Label or Dye: Yes
• Kit Type: 2-Step RT-qPCR system
• Label or Dye: Fluorescein (FITC)
• No. of Tests: 50
• Passive Reference Dye: CXR (Separate Tube)
• Product Line: GoTaq, GoScript
• Product Type: RT-qPCR Kit
• Quantity: 5mL
• Research Category: Gene Expression
• Reverse Transcriptase: GoScript™
• Ribonuclease H Activity: Yes
• Shipping Condition: Dry Ice
• Sufficient For: 50 RT Reactions and 500 x 20 μL qPCR Reactions
• For Use With (Application): Gene Expression
• Activation Time: 2 min.
• Compatibility: RT-PCR Instruments that Detect SYBR Green I or FAMTM Dye
• Final Product Type: First-Strand cDNA
• Hot Start: Built-In Hot Start
• No. of Reactions: 500 Reactions
• Reagent Type: RT-qPCR, cDNA Synthesis
• Sample Type: Blood, Cells, Feces, FFPE, Food, Human Cerebrospinal Fluid, Plants, Plasma, Saliva, Serum, Sputum, Swabs, Tissue, Urine
• Starting Material: RNA
• Volume: 20 μL
• Storage Buffer: 2X GoTaq™ qPCR Master Mix, 5X GoScript™ Reaction Buffer

• U.S. Patent 6,242,235, Australian Patent 761757, Canadian Patent 2,335,153 and other patents and patents pending.
• U.S. Patent 5,552,302.
• Licensed under U.S. Patents 5,338,671 and 5,587,287 and corresponding patents in other countries. For research use only. Not for use in diagnostic procedures.
• Notice to Purchaser: Limited License
• Use of this product is covered by one or more of the following U.S. patents and corresponding patent claims outside the U.S.: 5,789,224; 5,618,711; 6,127,155 and claims outside the U.S. corresponding to U.S. Patent 4,889,818. The purchase of this product includes a limited, nontransferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as the patented 5' Nuclease Process claims in U.S. Patents 5,210,015 and 5,487,972), no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.