Promega GoTaq™ DNA Polymerase

Taq DNA Polymerase. Provided with Green and Colorless Reaction Buffers. Green Buffer allows direct gel analysis after amplification. Enhanced performance over standard Taq DNA Polymerase.

Includes:

• GoTaq DNA Polymerase; 5X Colorless GoTaq Reaction Buffer (Used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction); 5X Green GoTaq Reaction Buffer (Contains two dyes that separate during electrophoresis to facilitate direct-to-gel analysis of PCR products). Both buffers are supplied at pH 8.5 and contain MgCl₂ at a concentration of 7.5mM, giving a final concentration of 1.5mM in a 1X reaction
• GoTaq Green Master Mix or GoTaq Colorless Master Mix, nuclease-free water

Catalog Number	Mfr. No.	Includes	Quantity
PRM3001	Promega M3001	GoTaq™ DNA Polymerase, 1mL 5X green GoTaq™ reaction Buffer and 1mL 5X colorless GoTaq™ reaction Buffer, MgCl2 at a concentration of 7.5mM, nuclease free water	100U
PRM3008	Promega M3008	GoTaq™ DNA Polymerase, 4 x 5mL 5X green GoTaq™ reaction Buffer and 5X colorless GoTaq™ reaction Buffer, MgCl2 at a concentration of 7.5mM, nuclease free water	2500U
PRM3005	Promega M3005	GoTaq™ DNA Polymerase, 4 x 1mL 5X Green GoTaq™ reaction Buffer and 4 x 1mL 5X colorless GoTaq™ reaction Buffer, MgCl2 at a concentration of 7.5mM, nuclease free water	500U

Description

• Taq DNA Polymerase with Buffers for Enhanced Amplification
• Direct-to-gel convenience
• Can be used with your current cycling conditions
• Supplied at a concentration of 5u/µL

The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Published Patent Appln. 20020150900 and U.S. Pat. Nos. 5,432,272, 6,617,106 and 6,140,496 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Licensing, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
This product is designed and sold for use in the multiplex PCR process covered by U.S. Pat. No. 5,582,989 and Canadian Pat. No. 1,339,731. A limited license has been granted under the patent to use only this amount of the product to practice the multiplex PCR process and is conveyed to the purchaser by the purchase of this product.

Specifications

• Concentration: 5U/μL
• For Use With (Application): PCR, two-step RT-PCR and T-vector cloning
• Purity: >90% Pure
• Storage Requirements: −30 to −10°C