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Promega GoTaq™ Probe 1-Step RT-qPCR System

Catalog No. PRA6120 Shop All Promega Corporation Products
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RT-qPCR System; Size: 200 reactions
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Catalog No.
PRA6120 RT-qPCR System; Size: 200 reactions
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Catalog No. PRA6120 Supplier Promega Supplier No. A6120
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Description

Includes

GoTaq™ Probe Master Mix with dUTP, CXR Reference Dye, GoScript™ RT Mix for 1-Step RT-qPCR and Nuclease-Free Water

GoTaq Probe RT-qPCR Systems enable detection and relative quantification of RNA expression levels.

  • Simple, One-Step RT-qPCR for RNA Expression Analysis
  • Includes GoScript™ Reverse Transcriptase and GoTaq(R) Probe qPCR Master Mix. RNasin(R) RNase protection provided in the supplied GoScript™ RT Mix
  • Compatible with fast and standard cycling methods using probe-based assays
  • Sensitive detection for quantitation over a broad dynamic range

Specifications

Calibration Type Hydrolysis Probe Calibration
Content And Storage Probe master mix with dUTP (2 x 1mL), CXR reference dye (1 x 200μL), RT mix for 1-Step RT-qPCR (1 x 225μL), Nuclease free Water (2 x 1250μL); −30 to −10°C
Detection Method TaqMan
Form Liquid
Format Kit
PCR Method 1-step RT-qPCR
Polymerase GoTaq Taq DNA Polymerase
Reaction Speed Fast or Standard
Technique RT-qPCR
DNase DNase-free
For Use With (Equipment) Real-time PCR (qPCR) Instruments
Genotyping Target RNA Expression Levels
High-throughput Compatibility High-throughput Compatible
Includes Label or Dye No
Kit Type Probe 1-step RT-qPCR system
Label or Dye Unlabeled
No. of Tests 200
Passive Reference Dye CXR (Separate Tube)
Product Line GoTaq
Product Type RT-qPCR Kit
Quantity 2mL
Research Category Gene Expression Analysis
Reverse Transcriptase GoScript™
Ribonuclease H Activity Yes
Shipping Condition Dry Ice
Sufficient For 200 Reactions
Target Organism Class Animal, Bacteria, Fungi, Human, Parasites, Viruses, Yeast
For Use With (Application) Real Time PCR (qPCR)
Activation Time 2 min.
Compatibility RT- PCR (qPCR)
Final Product Type cDNA
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Reaction Format Master Mix
Reagent Type RT-qPCR Master Mix
Sample Type Blood, Cells, Feces, FFPE, Food, Human Cerebrospinal Fluid, Plants, Plasma, Saliva, Serum, Sputum, Swabs, Tissue, Urine
Starting Material 10 pg to 1 μg RNA
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