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Promega GoTaq™ Probe 2-Step RT-qPCR System

Catalog No. PRA6110 Shop All Promega Corporation Products
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PRA6110 200 reactions
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Catalog No. PRA6110 Supplier Promega Supplier No. A6110
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Description

Robust system to simplify RNA expression analysis. The GoTaq™ Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels.

  • Robust System to Simplify RNA Expression Analysis
  • Integrated components for reverse transcription and downstream qPCR, including RNasin(R) Ribonuclease Inhibitor
  • Compatible with both fast and standard cycling methods using probe-based assays
  • Sensitive detection for quantitation over a broad dynamic range

Specifications

Calibration Type Hydrolysis Probe Calibration
Content And Storage 2X Probe qPCR master mix (2 x 1mL), PCR Nucleotide mix (1 x 200μL), 5X reaction Buffer (1 x 300μL), reverse Transcriptase (1 x 50μL), Oligo(dT)15 Primer 1 x 50μg; −30 to −10°C
Detection Method Primer-probe
Form Liquid
Format Kit
PCR Method 2-step RT-qPCR
Polymerase GoTaq™ Hot Start Polymerase
Reaction Speed Fast or Standard
Technique Reverse Transcription, RT-qPCR
DNase DNase-free
For Use With (Equipment) RT- PCR Systems
Genotyping Target Gene Expression
High-throughput Compatibility High-throughput Compatible
Includes Label or Dye Yes
Kit Type Probe 2-step RT-qPCR system
Label or Dye Unlabeled
No. of Tests 200
Optimal Reaction Temperature 95°C
Passive Reference Dye CXR (Separate Tube)
Primer Oligo(dT)15 Primer
Product Line GoTaq
Product Type RT-qPCR Kit
Quantity 2mL
Research Category Gene Expression Analysis
Reverse Transcriptase GoScript™
Ribonuclease H Activity Yes
Shipping Condition Dry Ice
Sufficient For 200 Reactions
For Use With (Application) Real Time PCR (qPCR)
Compatibility RT- PCR (qPCR)
Fidelity (vs. Taq) 1X
Final Product Type cDNA
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Reaction Format Separate Components
Reagent Type Reverse Transcription, qPCR
Sample Type Blood, Cells, Feces, FFPE, Food, Human Cerebrospinal Fluid, Plants, Plasma, Saliva, Serum, Sputum, Swabs, Tissue, Urine
Starting Material RNA
Source E. coli
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