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  7. Promega GoTaq™ Probe qPCR Master Mix  

Promega GoTaq™ Probe qPCR Master Mix

Catalog No. PRA6101 Shop All Promega Corporation Products
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No. of Reactions:
1000 Reactions
200 Reactions
Quantity:
2 mL
10 mL
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Catalog No. No. of Reactions Quantity
PRA6101 200 Reactions 2 mL
PRA6102 1000 Reactions 10 mL
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Catalog No. PRA6101 Supplier Promega Supplier No. A6101
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Description

GoTaq™ Probe qPCR Master Mix is a ready-to-use stabilized 2X formulation containing GoTaq™ Hot Start Polymerase, MgCl2, dNTPs and proprietary reaction buffer.

  • Convenient qPCR Amplification Using Hydrolysis Detection Probes
  • Ready-to-use mix of optimized components for robust amplification
  • Compatible with both fast and standard cycling methods
  • Sensitive detection for quantitation over a broad dynamic range

Specifications

Concentration 2X
Content And Storage • 2X GoTaq Probe qPCR Master Mix (dTTP), 2 x 1 mL
• 30μM CXR Reference Dye, 100 μL
• Nuclease-free Water, 2 x 1.25 mL

Store at –30° to –10°C.
Detection Method Primer-probe
Form Frozen
Format Liquid
PCR Method qPCR
Reaction Speed Fast or Standard
Technique qPCR
For Use With (Equipment) Real-Time PCR Systems
Kit Type QPCR Detection Kit
Optimal Reaction Temperature 60°C
Passive Reference Dye CXR Reference Dye (Separate Tube)
Quantity 2 mL
Shipping Condition Frozen
For Use With (Application) Gene Expression
Thermostability GoTaq DNA Polymerase activation: 95°C for 2 minutes, Denaturation: 95°C for 15 seconds (standard cycling) or 3 seconds (fast cycling), Annealing and Extension: 60°C
Compatibility Both standard and fast instrument cycling programs
Hot Start Antibody-mediated Hot-start Chemistry
No. of Reactions 200 Reactions
Sample Type DNA Template
Inhibitor Tolerance (Simple) High
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