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  7. Promega GoTaq™ Probe qPCR Master Mix  

Promega GoTaq™ Probe qPCR Master Mix

Catalog No. PRA6102 Shop All Promega Corporation Products
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No. of Reactions:
1000 Reactions
200 Reactions
Quantity:
2 mL
10 mL
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Catalog No. No. of Reactions Quantity
PRA6102 1000 Reactions 10 mL
PRA6101 200 Reactions 2 mL
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Catalog No. PRA6102 Supplier Promega Supplier No. A6102
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Description

GoTaq™ Probe qPCR Master Mix is a ready-to-use stabilized 2X formulation containing GoTaq™ Hot Start Polymerase, MgCl2, dNTPs and proprietary reaction buffer.

  • Convenient qPCR Amplification Using Hydrolysis Detection Probes
  • Ready-to-use mix of optimized components for robust amplification
  • Compatible with both fast and standard cycling methods
  • Sensitive detection for quantitation over a broad dynamic range

Specifications

Concentration 2X
Content And Storage • 2X GoTaq Probe qPCR Master Mix (dTTP), 10 x 1 mL
• 30μM CXR Reference Dye, 2 x 200 μL
• Nuclease-free Water, 13 mL

Store at –30° to –10°C.
Detection Method Primer-probe
Form Liquid
Format Ready-to-use, Stabilized 2X Formulation
PCR Method qPCR
Reaction Speed Fast or Standard
Technique Quantitative PCR (qPCR)
Optimal Reaction Temperature 95°C (GoTaq DNA Polymerase activation), 95°C (Denaturation), 60°C (Annealing and extension)
Passive Reference Dye Carboxy-X-rhodamine (CXR) Reference dye (30 μM)
Primer qPCR Primers
Quantity 10 mL
For Use With (Application) Gene Expression
Compatibility Both standard and fast instrument cycling programs
Hot Start Antibody-mediated Hot-start Chemistry
No. of Reactions 1000 Reactions
Inhibitor Tolerance (Simple) Provides Resistance to a wide range of PCR Inhibitors
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