Promega MagneHis™ Protein Purification System

Catalog No. PR-V8500

Supplier: Promega V8500

Description

Includes

MagneHis binding/wash buffer, 50mL; FastBreak™ cell lysis reagent, 15mL; DNase I, lyophilized, 1 vial; MagneHis elution buffer, 10mL; MagneHis Ni-particles, 2mL

The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.

• Magnetic Purification of Polyhistidine- or HQ-tagged Proteins from E. coli or Mammalian Cell Lysates
• No centrifugation or vacuum required after cell lysis
• Particles are compatible with a variety of common buffers
• Binding capacity up to 1mg of polyhistidine-tagged protein per 1mL of particles

• U.S. Pat. Nos. 7,112,552; 7,304,015; 7,304,016; 7,354,750; 6,174,704; and 7,319,021 and other patents and patents pending.
• Certain applications of this product are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

• Kit Contents: 50mL MagneHis binding/Wash Buffer, 15mL FastBreak™ cell lysis reagent, DNase I, 1 vial lyophilized, 10mL MagneHis Elution Buffer, 2mL MagneHis Ni-particles
• Content And Storage: 2°C to 10°C
• Quantity: 65 Reactions
• For Use With (Application): Rapid and reliable purification of Polyhistidine-tagged Proteins from 1mL to 1L of bacterial culture
• Type: Magnetic Bead Protein Purification System