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Promega pGL3 Luciferase Reporter Vectors
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Vector:
pGL3-basic vector
pGL3-control vector
pGL3-enhancer vector
pGL3-promoter vector
Description
Includes
Vector DNA, 20μg; Bacterial strain JM109, 500μL
- Four Reporter Vectors That Contain luc+, a Modified Firefly Luciferase Gene
- Unique NcoI site located at 5′ end of luc+ gene will create fusions with reporter gene
- SmaI site in the MCS means blunt-ended inserts can be ligated into the MCS and cut on either side by other restriction endonucleases
- XbaI site just downstream of luc+ gene facilitates insertions into the 3′ untranslated region of mRNA or subcloning of the luciferase gene
- U.S. Patent 5,670,356.
- The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Specifications
Specifications
Promoter | SV40 |
Reporter Gene | Luciferase |
Content And Storage | -30°C to -10°C |
Quantity | 20 μg |
Vector | pGL3-control vector |
For Use With (Application) | Quantitative analysis of factors that potentially regulate mammalian gene expression |
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