Promega pGL3 Luciferase Reporter Vectors

Description

Includes

Vector DNA, 20μg; Bacterial strain JM109, 500μL

Four firefly luciferase reporter vectors.

• Four Reporter Vectors That Contain luc+, a Modified Firefly Luciferase Gene
• Unique NcoI site located at 5′ end of luc+ gene will create fusions with reporter gene
• SmaI site in the MCS means blunt-ended inserts can be ligated into the MCS and cut on either side by other restriction endonucleases
• XbaI site just downstream of luc+ gene facilitates insertions into the 3′ untranslated region of mRNA or subcloning of the luciferase gene

1. U.S. Patent 5,670,356.
2. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

Specifications

• Promoter: SV40
• Reporter Gene: Luciferase
• Content And Storage: -30°C to -10°C
• Quantity: 20 μg
• Vector: pGL3-promoter vector
• For Use With (Application): Quantitative analysis of factors that potentially regulate mammalian gene expression