Promega Protease-Glo™ Assay

Description

Includes

2X Rapid Ligation Buffer, 200μL; Oligo Annealing Buffer, 1mL; Bright-Glo™ Luciferase Assay Substrate, 1 vial; Bright-Glo Luciferase Assay Buffer, 10mL; TNT™ SP6 High-Yield Wheat Germ Master Mix, 300μL; T4 DNA ligase, 100U; DTT, 250μL; Nuclease-free water, 1.25mL; 20X ProTEV Buffer, 1mL; ProTEV Protease, 1000U; pGloSensor™-10F Linear Vector, 20μL; pGloSensor-10F(TEV) Control Plasmid, 100μL

The Protease-Glo™ Assay is a novel method to detect and measure protease activities using a genetically engineered firefly (Photinus pyralis) luciferase and represents one example of the GloSensor platform technology.

• Detect and Measure Protease Activities
• Luminescent assay
• Quickly create your own protease substrates
• Easy quantitation, high sensitivity and wide dynamic range

Specifications

• For Use With (Application): Ideal for determining optimal Protease recognition sequences or effects of Amino Acid substitutions
• Includes: 200μL 2X Rapid Ligation Buffer, 1mL Oligo Annealing Buffer, 1 vial Luciferase Assay Substrate, 10mL Bright Glo Luciferase Assay Buffer, 300μL TnT™ SP6 High Yield Wheat Germ Master Mix
• Content And Storage: <−65°C
• Product Type: Protease Glo assay
• Quantity: Each

Not for use in assaying luminescence in living cells or animals. For use in living cells or animals contact Promega regarding the availability of licenses. For research use only. The terms of the limited license conveyed with the purchase of this Product are as follows. Researchers may use this Product, and its derivatives, modifications, components and progeny (collectively, the Derivatives) in their own research solely for the extracellular (in vitro) detection or determination of protease activity. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the circularly permuted luciferase reporter (Reporter) of the Product and Derivatives except that researchers may clone heterologous DNA sequences via addition of oligonucleotides at the site corresponding to the free ends of the linearized Product. In addition, researchers must either: (1) use reagents purchased from Promega Corporation for all (a) determinations or detections of luminescence activity, (b) extracellular transcription of DNA sequences, and (c) extracellular translation of transcripts, resulting from the research use of the Product and Derivatives; or (2) contact Promega to obtain a license for any and all other use of the Product and Derivatives including, without limitation, use in conjunction with reagents not purchased from Promega. For use of this product for purposes other than protease determination or detection, contact Promega for written authorization. Researchers may transfer Derivatives to others provided that recipients agree to be bound by the conditions of this label license prior to such transfer. No reach-through payments shall be owed to Promega relating to an organization's commercialization of products that are the discoveries resulting from the research use of this Product or Derivatives, provided that such products of the organization do not fall within the scope of the valid claims of any issued patents assigned or licensed to Promega, or that such commercialization would not be a violation of the terms of this label license. No other use of this Product or Derivatives is authorized without the express written consent of Promega. If the purchaser/recipient is not willing to accept the conditions of this limited use statement, Promega will accept the return of the unopened Product and provide the purchaser/recipient with a full refund. However, in the event this Product is opened, then the purchaser/recipient agrees to be bound by the conditions of this limited use statement.

Australian Pat. No. 2001 285278 and other patents pending.

The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

Licensed for research and laboratory use only under U.S. Patent 5,547,862.

Licensed under U.S. Patent 5,910,628.