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Promega T4 DNA Ligase
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Quantity:
100 U
500 U
Concentration:
1 to 3 U/μL
10 to 20 U/μL
Description
- Joins Two DNA Strands with Cohesive or Blunt Ends
- Use with 5′, 3′ or blunt-ended dsDNA (e.g., inserts and vectors)
- Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
- Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
Specifications
Specifications
Concentration | 1 to 3 U/μL |
Color | Colorless |
Components | 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP |
Purity | >90% |
pH | 7.4 |
For Use With (Application) | Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts) |
Storage Buffer | 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol |
Content And Storage | -30°C to -10°C |
Source | Recombinant E. coli strain |
Quantity | 100 U |
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