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Promega T4 DNA Polymerase

Catalog No. PRM4211 Shop All Promega Corporation Products
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100 U
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PR-M4211 100 U
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Description

High-fidelity polymerase useful for mutagenesis reactions, 3'-overhang removal and 5'-overhang fill-in reactions.

  • Synthesizes DNA 5′→3′ from a Primed ssDNA Template
  • High fidelity enzyme of choice for applications where misincorporation is a concern
  • Flexible polymerase used in a variety of molecular applications; active in many Promega 1X restriction enzyme buffers
  • Heat inactivated by heating at 75°C for 10 minutes
  • Supplied at a concentration of 5-10u/μL

Specifications

Concentration 5 to 10 U/μL
Color Colorless
Components 10X Reaction Buffer: 250mM Tris acetate (pH 7.7), 1M Potassium Acetate, 100mM Magnesium Acetate and 10mM DTT
Purity >95%
pH 6.5
For Use With (Application) Flush 5' protruding ends with labeled or unlabeled dNTPs, blunt 3' overhangs, in-vitro mutagenesis
Storage Buffer 200mM Potassium Phosphate (pH 6.5 at 25°C), 2mM DTT, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 100 U
Product Type T4 DNA Polymerase
Molecular Weight (g/mol) 112
Form Liquid
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