Promega T4 Polynucleotide Kinase

Description

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

• Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides
• Can be inactivated by heating at 68°C for 10 minutes
• Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
• Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
• Supplied at a concentration of 5-10u/μL

Specifications

• Concentration: 5 to 10 U/μL
• Color: Colorless
• Components: 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
• Purity: ≥90%
• pH: 7.5
• For Use With (Application): 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
• Storage Buffer: 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Recombinant E. coli strain
• Quantity: 100 U
• Product Type: T4 Polynucleotide Kinase
• Molecular Weight (g/mol): 132
• Inhibitors: Ammonium Ions and low levels of Phosphate Buffers
• Form: Liquid