Invitrogen™ PSD-95 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 516900

Catalog No. 51-690-0

Description

This antibody recognizes the 95 kDa PSD-95 protein. Cross-reactivity with an unknown protein at ~50 kDa has been observed in western blots with rat brain homogenates. Western blots with mouse brain homogenates did not show such a band. Positive reactivity of this antibody was confirmed on western blots of rat brain homogenates and lysates of PSD-95- transfected HEK293 (human embryonic kidney) cells.

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Specifications

• Antigen: PSD-95
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DLG4
• Gene Alias: discs large homolog 4; discs large MAGUK scaffold protein 4; discs, large homolog 4; discs, large homolog 4 (Drosophila); disks large homolog 4; Dlg4; Dlgh4; FLJ97752; FLJ98574; post synaptic density; postsynaptic density protein 95; post-synaptic density protein 95; PSD95; PSD-95; PSD-95 alpha 2b; PSD-95 beta; Sap90; SAP-90; SAP90A; Synapse-associated protein 90; synapse-associated protein SAP90; Tax interaction protein 15
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13385, 1742, 29495
• Content And Storage: -20°C
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.25 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene Accession No.: P31016, P78352, Q62108
• Gene Symbols: DLG4
• Immunogen: KLH-conjugated synthethic peptide derived from the C-terminal region of human PSD-95.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG