R&D Systems™ SUMO1 Conjugation Kit

Description

Sufficient for 5 to 10 x 20uL reactions

The ubiquitin-like SUMO1 is conjugated to a variety of proteins in the presence of Ubc9 and the SUMO E1 activating enzyme (SAE1/SAE2 in human, or Aos1/Uba2p in yeast). Unlike SUMO2 and SUMO3, SUMO1 does not readily form poly-SUMO chains on substrates in vitro, though there is growing evidence that SUMO1 may cap or terminate poly-SUMO2/3 chains. The heterodimeric SAE1/SAE2 complex (38 and 70 kDa respectively) uses ATP to adenylate the C-terminal glycine residue of a SUMO protein; in a second step a thioester bond is formed between SUMO and a cysteine residue within the SAE2 subunit. Following this activation, SUMO is transferred to Cys93 of Ubc9. Ubc9 (also known as UBE2I) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it sometimes interacts directly with protein substrates that are SUMOylated, and can play a role in substrate recognition. SUMOylated substrates are often localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein function and stability.

Specifications

• Content And Storage: Store the unopened product at -70° C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Quantity: 1 Kit