RB1 Antibody (1F8), Alexa Fluor™ 750, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP254483AF750

Catalog No. NP24483A70

Description

RB1 Monoclonal specifically detects RB1 in Human, Mouse samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: RB1
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: RB1
• Immunogen: Recombinant human RB1 protein fragment of 283 amino acid residues. (Uniprot: P06400)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Clone: 1F8
• Dilution: Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin
• Gene Alias: OSRC, osteosarcoma, p105-Rb, pp110, pRb, Rb, retinoblastoma 1, retinoblastoma suspectibility protein, retinoblastoma-associated protein
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Research Discipline: Cancer, Cell Cycle and Replication, Phospho Specific, Transcription Factors and Regulators, Tumor Suppressors
• Gene ID (Entrez): 5925.0
• Target Species: Human, Mouse
• Isotype: IgG1 κ