Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase Available on GSA/VA Contract for Federal Government customers only.

A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.

Manufacturer:  Thermo Scientific™ EP0452

 View more versions of this product

Catalog No. FEREP0452


Add to cart

Description

Description

RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

  • High yields of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42 to 45°C
  • Active up to 55°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

Specifications

First strand cDNA synthesis for RT-PCR and real-time RT-PCR (3, 4, 5); Reverse transcription at elevated temperatures to reduce effects of secondary structure; Synthesis of cDNA for cloning and expression
5 x 1mL 5X Reaction Buffer
RevertAid H Minus Reverse Transcriptase
100U
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit