Rhodopsin Antibody (Rho 1D4), Alexa Fluor™ 647, Novus Biologicals™

Rabbit Monoclonal Antibody

Supplier:  Novus Biologicals NBP281059AF647

Catalog No. NB015098

Description

Rhodopsin Monoclonal specifically detects Rhodopsin in Human, Mouse, Rat, Amphibian, Bovine, Zebrafish samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: Rhodopsin
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Formulation: 50mM Sodium Borate
• Gene Symbols: RHO
• Immunogen: Bleached bovine rod outer segment (ROS).
• Quantity: 0.1 mL
• Research Discipline: GPCR, Phospho Specific, Signal Transduction, Vision
• Gene ID (Entrez): 6010
• Target Species: Human, Mouse, Rat, Amphibian, Bovine, Zebrafish
• Form: Purified

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Clone: Rho 1D4
• Dilution: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence
• Gene Alias: CSNBAD1, MGC138309, OPN2MGC138311, opsin 2, rod pigment, opsin-2, retinitis pigmentosa 4, autosomal dominant, rhodopsin, RP4
• Host Species: Rabbit
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
• Content And Storage: Store at 4°C in the dark.
• Isotype: IgG κ