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  6. Rhodopsin, Rabbit anti-Human,Mouse,Rat,Amphibian,Bovine,Zebrafish, Clone: Rho 1D4, Novus Biologicals™

Rhodopsin, Rabbit anti-Human,Mouse,Rat,Amphibian,Bovine,Zebrafish, Clone: Rho 1D4, Novus Biologicals™
SDP

Catalog No. NB015091 Shop All R&D Systems Products
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0.025 mg
0.2 mg
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NB015091 0.025 mg
NB015092 0.2 mg
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Catalog No. NB015091 Supplier Novus Biologicals Supplier No. NBP2810590.025MG
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Description

Rabbit Monoclonal Antibody

Rhodopsin Monoclonal specifically detects Rhodopsin in Human, Mouse, Rat, Amphibian, Bovine, Zebrafish samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay.

Specifications

Antigen Rhodopsin
Applications Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmune Assays (RIA)
Classification Monoclonal
Clone Rho 1D4
Concentration 1 mg/mL
Conjugate Unconjugated
Dilution Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay
Formulation PBS
Gene Alias CSNBAD1, MGC138309, OPN2MGC138311, opsin 2, rod pigment, opsin-2, retinitis pigmentosa 4, autosomal dominant, rhodopsin, RP4
Gene Symbols RHO
Host Species Rabbit
Immunogen Bleached bovine rod outer segment (ROS).
Purification Method Protein A purified
Quantity 0.025 mg
Regulatory Status RUO
Research Discipline GPCR, Phospho Specific, Signal Transduction, Vision
Primary or Secondary Primary
Gene ID (Entrez) 6010
Test Specificity The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Target Species Human, Mouse, Rat, Amphibian, Bovine, Zebrafish
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG κ
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