Promega Riboprobe™ Systems

Description

Includes

RNA polymerase(s); pGEM™ Express Positive Control Template; RQ1 RNase-free DNase; Recombinant RNasin™ Ribonuclease Inhibitor; rATP; rCTP; rGTP; rUTP; DTT; Transcription-optimized buffer, 5X; Nuclease-free water

The Riboprobe Systems are designed to prepare high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.

• Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts
• Systems available with SP6 RNA Polymerase, T7 RNA Polymerase or T3 RNA Polymerase
• SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
• Allow synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct

• U.S. Patent 5,552,302; Australian Patent 646803 and other patents.
• Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.

Specifications

• Content And Storage: Store at -20°C.
• For Use With (Application): Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA
• Includes: 500U RNA Polymerase (T7), 5μg pGEM™ express positive control template, 500μL transcription optimized 5X Buffer, 2500U recombinant RNasin™ RNase inhibitor, 100mM 100
• Product Type: Riboprobe System—T7
• Quantity: 1 System