RNA Polymerase II/POLR2A Antibody (4H8), Alexa Fluor™ 750, Novus Biologicals™

Catalog No.	Quantity
NB20598A750	0.1 mL

Catalog No. NB20598A750 Supplier Novus Biologicals Supplier No. NB200598AF750

Description

Mouse Monoclonal Antibody

RNA Polymerase II/POLR2A Monoclonal specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular).

Specifications

• Antigen: RNA Polymerase II/POLR2A
• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
• Classification: Monoclonal
• Clone: 4H8
• Conjugate: Alexa Fluor 750
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Alias: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
• Gene Symbols: POLR2A
• Host Species: Mouse
• Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProtNo. P24928]
• Purification Method: Protein G purified
• Quantity: 0.1 mL
• Research Discipline: Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators
• Primary or Secondary: Primary
• Gene ID (Entrez): 5430.0
• Test Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.′
• Target Species: Human, Mouse, Yeast, Primate
• Content And Storage: Store at 4°C in the dark.
• Isotype: IgG1