RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 9 publications

Supplier:  Novus Biologicals NB200598SS

Catalog No. NB200598SS

Description

RNA Polymerase II/POLR2A Monoclonal specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Flow (Intracellular), CyTOF-ready.

Specifications

• Antigen: RNA Polymerase II/POLR2A
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot 1-2 ug/ml, Flow Cytometry 1 ug per million cells, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:10-1:500, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready
• Gene Alias: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
• Host Species: Mouse
• Molecular Weight of Antigen: 217 kDa
• Quantity: 0.025 mL
• Research Discipline: Chromatin Research, Epigenetics, microRNA, Transcription Factors and Regulators
• Gene ID (Entrez): 5430
• Target Species: Human, Mouse, Yeast, Primate (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
• Clone: 4H8
• Conjugate: Unconjugated
• Gene Accession No.: P24928
• Gene Symbols: POLR2A
• Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.'
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1

For Research Use Only