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  6. ROCK1 Antibody (154C1465) - (active/cleaved) - BSA Free, Novus Biologicals™

ROCK1 Antibody (154C1465) - (active/cleaved) - BSA Free, Novus Biologicals™
SDP

Catalog No. NB10056596 Shop All R&D Systems Products
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0.1 mg
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NB10056596 0.1 mg
NB10056596T 0.025 mg
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Catalog No. NB10056596 Supplier Novus Biologicals Supplier No. NB10056596
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Description

Mouse Monoclonal Antibody has been used in 5 publications

cleaved ROCK1 Monoclonal specifically detects cleaved ROCK1 in Canine, Human, Mouse, Primate, Rabbit, Rat samples. It is validated for Immunocytochemistry/Immunofluorescence, Western Blot.

Specifications

Antigen cleaved ROCK1
Applications Immunocytochemistry, Immunofluorescence, Western Blot
Classification Monoclonal
Clone 154C1465
Concentration 0.5 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1-2 ug/ml, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 21059388)
Gene Accession No. O60259
Gene Alias EC 2.7.11, EC 2.7.11.1, MGC131603, MGC43611, p160 ROCK-1, p160ROCK, p160-ROCK, PRO0435, Renal carcinoma antigen NY-REN-35, Rho kinase, rho-associated protein kinase 1, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1
Gene Symbols ROCK1
Host Species Mouse
Immunogen This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Purification Method Protein G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Apoptosis, Breast Cancer, Cancer, Signal Transduction, Wnt Signaling Pathway
Primary or Secondary Primary
Gene ID (Entrez) 6093
Test Specificity This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Target Species Canine, Mouse, Human, Mouse, Primate, Rabbit, Rabbit, Rat
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG
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