ROCK1 Antibody (154C1465) - (active/cleaved) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 5 publications

Supplier:  Novus Biologicals NB10056596

Catalog No. NB10056596

Description

ROCK1 Monoclonal specifically detects ROCK1 in Human, Mouse, Rat, Canine, Primate, Rabbit samples. It is validated for Western Blot, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: cleaved ROCK1
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Dilution: Western Blot 1-2 ug/ml, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 21059388)
• Gene Alias: EC 2.7.11, EC 2.7.11.1, MGC131603, MGC43611, p160 ROCK-1, p160ROCK, p160-ROCK, PRO0435, Renal carcinoma antigen NY-REN-35, Rho kinase, rho-associated protein kinase 1, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Clone: 154C1465
• Conjugate: Unconjugated
• Gene Accession No.: O60259
• Gene Symbols: ROCK1
• Immunogen: This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Signal Transduction, Wnt Signaling Pathway
• Gene ID (Entrez): 6093
• Target Species: Human, Mouse, Rat, Canine, Primate, Rabbit
• Form: Purified

For Research Use Only