ROCK1 Antibody (154C1465) - (active/cleaved) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 5 publications

Catalog Number	Mfr. No.	Quantity
NB10056596T	Novus Biologicals NB10056596SS	0.025 mg
NB10056596	Novus Biologicals NB10056596	0.1 mg

Description

ROCK1 Monoclonal specifically detects ROCK1 in Human, Mouse, Rat, Canine, Primate, Rabbit samples. It is validated for Western Blot, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: cleaved ROCK1
• Concentration: 0.5 mg/mL
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Signal Transduction, Wnt Signaling Pathway
• Gene Accession No.: O60259
• Gene ID (Entrez): 6093
• Immunogen: This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 154C1465
• Dilution: Western Blot 1-2 ug/ml, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 21059388)
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Canine, Primate, Rabbit
• Gene Alias: EC 2.7.11, EC 2.7.11.1, MGC131603, MGC43611, p160 ROCK-1, p160ROCK, p160-ROCK, PRO0435, Renal carcinoma antigen NY-REN-35, Rho kinase, rho-associated protein kinase 1, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1
• Gene Symbols: ROCK1
• Isotype: IgG
• Purification Method: Protein G purified
• Test Specificity: This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.

For Research Use Only