SerpinB9 Antibody (7D8), Alexa Fluor™ 532, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB10064359AF532

Catalog No. N106439A32

Description

SerpinB9 Monoclonal specifically detects SerpinB9 in Human, Mouse (Negative), Porcine (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: SerpinB9
• Classification: Monoclonal
• Conjugate: Alexa Fluor 532
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: SERPINB9
• Immunogen: Recombinant PI-9 produced in P. pastoris.
• Primary or Secondary: Primary
• Test Specificity: NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: 7D8
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
• Host Species: Mouse
• Quantity: 0.1 mL
• Gene ID (Entrez): 5272
• Target Species: Human, Mouse, Pig
• Isotype: IgG1