SerpinB9 Antibody (7D8), Alexa Fluor™ 647, Novus Biologicals™

Catalog No.	Quantity
NB10064359Y	0.1 mL

Catalog No. NB10064359Y Supplier Novus Biologicals Supplier No. NB10064359AF647

Description

Mouse Monoclonal Antibody

SerpinB9 Monoclonal specifically detects SerpinB9 in Human, Mouse (Negative), Porcine (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot.

Specifications

• Antigen: SerpinB9
• Applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 7D8
• Conjugate: Alexa Fluor 647
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Accession No.: P50453
• Gene Alias: CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
• Gene Symbols: SERPINB9
• Host Species: Mouse
• Immunogen: Recombinant PI-9 produced in P. pastoris.
• Purification Method: Protein G purified
• Quantity: 0.1 mL
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Immunology
• Primary or Secondary: Primary
• Gene ID (Entrez): 5272
• Test Specificity: NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).
• Target Species: Human, Mouse (Negative), Pig (Negative)
• Content And Storage: Store at 4C in the dark.
• Form: Purified
• Isotype: IgG1

For Research Use Only