Siglec-1/CD169 Antibody (ED3) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB017705	Novus Biologicals NB100653010.025MG	0.025 mg
NB10065301	Novus Biologicals NB10065301	0.1 mg

Description

Siglec-1/CD169 Monoclonal specifically detects Siglec-1/CD169 in Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen.

Specifications

• Antigen: Siglec-1/CD169
• Concentration: 0.5 mg/mL
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Rat
• Gene ID (Entrez): 6614
• Immunogen: Rat Spleen cell homogenate
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: ED3
• Dilution: Flow Cytometry 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:50-1:250
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Gene Alias: CD169, FLJ00051, sialic acid binding Ig-like lectin 1, sialoadhesin, sialoadhesin
• Gene Symbols: SIGLEC1
• Isotype: IgG2a
• Purification Method: Protein A purified
• Test Specificity: NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.

For Research Use Only